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Primer3 | Input

PRIMER_MIN_SIZE=18 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=25

Next time you are frustrated that "no primers were found," don't tweak the sequence—. Loosen the Tm range by 1°C or extend the product size range. The perfect primer pair is always just a parameter change away. Have a tricky multiplexing scenario? Drop the parameters in the comments below. primer3 input

PRIMER_PRODUCT_SIZE_RANGE=150-250 PRIMER_PRODUCT_OPT_SIZE=200 primer3 input

If you have ever used an online primer design tool, chances are you were using under the hood. Written in C and later wrapped in Python (Primer3-py) or web interfaces (Primer3Plus), this engine is the gold standard for picking oligonucleotides. primer3 input